The 321 series is a direct current commuter train that began operation in December 2005. It is primarily utilized on the JR Kyoto Line and JR Kobe Line (Tokaido and Sanyo Main Lines) and the JR Takarazuka Line (Fukuchiyama Line). With the opening of the JR Tozai Line in 2008, the train also services the Gakkentoshi Line (Katamachi Line) and operates through the Yamatoji Line (Kansai Main Line) via the Osaka East Line, making it active across a broad range of the Kansai region.
- Compatible with interior lighting kit Kato 11-211 (11-212)